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KMID : 1098420060140040225
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Korean Journal of Medicinal Crop Science
2006 Volume.14 No. 4 p.225 ~ p.228
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Effects of Plant Growth Regulation on Adventitious Root Formation of Pulsatilla koreana Nakai
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Yoon Eui-Soo
Kwon Hye-Kyoung
Cho Yi-Yun
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Abstract
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To acquire the normal regeneration of plantlets, we investigated combinations and concentrations of plant growth regulations for optimal conditions of adventitious root formation. Based on the previous study, we performed callus and shoot induction. When induced shoot was transferred into a rooting medium containing plant hormones, it wilted and died. Thus, the shoot proliferated on 1/2 MS medium for 10 days and was then treated with MS medium supplemented with 3.0 mg/L NAA for 3 days. Adventitious root formations were observed after shoot planlets were transferred to 1/3 MS medium. The concentrations of salt and sucrose were gradually reduced in MS medium and the rooted plantlets were transferred for acclimatization into a mixture of peatmoos : perlite (3 : 2).
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KEYWORD
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plantlet regeneration, adventitious root formation, Pulsatilla koreana Nakai
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